刘龙奇

杭州华大生命科学研究院执行院长

刘龙奇，深圳华大生命科学研究院博士，杭州华大生命科学研究院执行院长、华大生命科学研究院细胞组学领域首席科学家。

人物经历

2015年，博士毕业并加入华大

2019年，深圳华大生命科学研究院的研究团队研发出了同时捕获单细胞内染色质可及性和转录组的测序技术（scCAT-seq）研究人员刘龙奇表示：“如何在单个细胞中充分获得多种维度的信息是目前的重要挑战，该技术最大的意义在于在一个单细胞里同时获得了染色质结构和基因表达双重信息。通过开发算法对这两种信息的整合，我们现在能够在单细胞水平看到很多过去人们未知的信息，比如单细胞水平DNA元件对靶基因的调控关系。”

2022年3月，中国科学院和深圳华大生命科学研究院等多家机构的研究者，通过体细胞诱导培养出了类似受精卵发育3天状态的人类全能干细胞。相关研究成果于北京时间3月22日凌晨在国际学术期刊《自然》（Nature）上发表。论文的另一位通讯作者、深圳华大生命科学研究院刘龙奇博士介绍，“通过大规模单细胞多组学图谱的方法，对干细胞技术手段在体外或体内获得的细胞或组织进行高效鉴定和机制解析，将极大地加速再生医学领域的发展。”

2022年4月，华大联合国内外科研机构绘制的全球首个非人灵长类动物全细胞图谱，于北京时间4月13日晚，发表于国际顶级学术期刊《自然》（Nature）。刘龙奇在接受澎湃新闻记者专访时表示，现阶段而言，人类细胞图谱的全面解析仍存在多项挑战，他们近年来将目光聚焦到和人的基因相似度高达93%的食蟹猕猴（Macaca fascicularis）。

2022年5月4日晚，深圳华大生命科学研究院联合多家机构在Cell出版社官网以时空组学联盟（STOC）专题的形式发布了全球首批生命时空图谱。这是首次从时间和空间维度上对生命发育过程中的基因和细胞变化过程进行超高精度解析，为认知器官结构、生命发育、人类疾病和物种演化提供全新方向。

“时空组学技术的出现，实现了生命在时间和空间维度上细胞‘地图’的全面绘制，让我们可以在细胞甚至亚细胞分辨率下，观察到正常状态和疾病状态下分子和细胞的分布及细胞之间的互作情况，真正实现了从高精度结构的角度去理解功能，这将大大推动我们对于生命复杂性和人类疾病的全面认知。”系列论文的共同通讯作者之一、深圳华大生命科学研究院单细胞组学首席科学家刘龙奇表示。

发表论文

1. Xu Y, Liu L#, Laslett A L, Miguel Esteban#.Cell reprogramming: into the groove. Nature Materials, 2013,12(12): 1082-1084. (#Co-corresponding authors)

2. Liu L, Xu Y, HeM, et al. Transcriptional pause release is a rate-limiting step for somaticcell reprogramming. Cell Stem Cell, 2014, 15(5): 574-588

3. Liu L, Leng L,Liu C, et al. An integrated chromatin accessibility and transcriptomelandscape of human pre-implantation embryos[J]. Nat Commun 10, 364 (2019)

4. Liu L, Liu C,Quintero A, et al. Deconvolution of single-cell multi-omics layers revealsregulatory heterogeneity[J]. Nat Commun 10, 470 (2019)

5. Liu C, Wang M, Wei X, …, Liu L#. An ATAC-seq atlas of chromatin accessibility in mousetissues[J]. Scientific data, 2019, 6(1): 1-10. (#Correspondingauthor)

6. Y, Sun N, Li C, …, Liu L, et al. Dissecting primateearly post-implantation development using long-term in vitro embryo culture[J].Science, 2019, 366(6467).

7. Zhu L, Yang P, Zhao Y, Zhuang Z, Wang Z, Song R, Zhang J, …, Wang F#, Gao GF#, Liu L#, Liu WJ#. (2020), Single-cellsequencing of peripheral mononuclear cells reveals distinct immuneresponse landscapes of COVID-19 and influenza patients, Immunity,2020 Sep 15;53(3):685-696. (#Corresponding authors)

8. Li, H., Wei, X., Zhou, L. ,…, Liu L,et al. Dynamiccell transition and immune response landscapes of axolotl limb regenerationrevealed by single-cell analysis. Protein Cell 12, 57–66(2021).

9. Yu Y, Wei X, Deng Q, Lan Q, Guo Y, Han L, Yuan Y, Fan P, Wu P, ShangguanS, Liu Y, Lai Y, Volpe G, Esteban MA, Liu C, Hou Y# and Liu L# .Single-Nucleus ChromatinAccessibility Landscape Reveals Diversity in Regulatory Regions Across DistinctAdult Rat Cortex. Front Mol Neurosci. 2021 May17;14:651355

10. Yuan Y, Deng Q, Wei X, Liu Y, Lan Q, Jiang Y, Yu Y, Guo P, Xu J, Yu C,Han L, Cheng M, Wu P, Zhang X, Lai Y, Volpe G, Esteban MA, Yang H, Liu C# and Liu L# (2021) The Chromatin Accessibility Landscape of Adult Rat. Front Genet. 2021May 24;12:651604

11. LiY, Yang T, Lai T, You L, Yang F, Qiu J, Wang L, Du W, Hua C, Xu Z, Cai J, Li Z,Liu Y, Li L, Zhang M, Chen J, Zhang L, Chen D, Wang W, Liu S, Wu L, Zeng W,Wang B, Wei X#, Liu L#,Chen F#.CDCP: A visualization and analyzing platform for single-cell datasets. J Genet Genomics. 2021 Dec 29:S1673-8527(21)00374-X. doi:10.1016/j.jgg.2021.12.004. Online ahead of print.

12. Mazid, M.A., Ward, C., Luo, Z., .Liu,L#., et al. Rolling back ofhuman pluripotent stem cells to an 8-cell embryo-like stage. Nature. 2022May;605(7909):315-324. doi: 10.1038/s41586-022-04625-0. Epub 2022 Mar21.PMID: 35314832

13. ChenA, Liao S, Cheng M, Ma K, Wu L, Lai Y, Qiu X, Yang J, Xu J, Hao S, Wang X, LuH, Chen X, Liu X, Huang X, Li Z, Hong Y, Jiang Y, Peng J, Liu S, Shen M, Liu C,Li Q, Yuan Y, Wei X, Zheng H, Feng W, Wang Z, Liu Y, Wang Z, Yang Y, Xiang H,Han L, Qin B, Guo P, Lai G, Muñoz-Cánoves P, Maxwell PH, Thiery JP, Wu QF, ZhaoF, Chen B, Li M, Dai X, Wang S, Kuang H, Hui J, Wang L, Fei JF, Wang O, Wei X,Lu H, Wang B, Liu S, Gu Y, Ni M, Zhang W, Mu F, Yin Y, Yang H, Lisby M, CornallRJ, Mulder J, Uhlén M, Esteban MA#, Li Y#, Liu L#, XuX#, Wang J#. Spatiotemporal transcriptomic atlas ofmouse organogenesis using DNA nanoball-patterned arrays. Cell. 2022 May12;185(10):1777-1792.e21. doi: 10.1016/j.cell.2022.04.003. Epub 2022 May 4.PMID: 35512705

14.Jiang Y, Hao S, Chen X,Cheng M, Xu J, Li C, Zheng H, Volpe G, Chen A, Liao S, Liu C#, Liu L#, Xu X#. Spatial Transcriptome Uncovers theMouse Lung Architectures and Functions. Front Genet. 2022 Mar 9;13:858808. doi:10.3389/fgene.2022.858808. eCollection 2022.PMID: 35391793

15.Han L, Wei X, Liu C, HouY#, Xu X#, Esteban MA#, Liu L#, et al.. Cell transcriptomic atlas ofthe non-human primate Macaca fascicularis.Nature. 2022 Apr;604(7907):723-731. doi:10.1038/s41586-022-04587-3. Epub 2022 Apr 13.PMID: 35418686

16. Liu X, Jiang Y, Song D, Zhang L, Xu G, Hou R,Zhang Y, Chen J, Cheng Y, Liu L, Xu X, Chen G, Wu D, Chen T, ChenA, Wang X. Clinical challenges of tissue preparation forspatial transcriptome. Clin Transl Med. 2022 Jan;12(1):e669.doi: 10.1002/ctm2.669.PMID: 35083877

17.Wang M, Hu Q, Lv T, WangY, Lan Q, Xiang R, Tu Z, Wei Y, Han K, Shi C, Guo J, Liu C, Yang T, Du W, An Y,Cheng M, Xu J, Lu H, Li W, Zhang S, Chen A, Chen W, Li Y#, Wang X#, Xu X#, HuY#, Liu L#. High-resolution 3Dspatiotemporal transcriptomic maps of developing Drosophila embryos and larvae .Dev Cell.2022 Apr 17:S1534-5807(22)00246-5. doi: 10.1016/j.devcel.2022.04.006. Onlineahead of print.PMID: 35512700

18.Xu J, Hao S, Shi Q, Deng Q, Jiang Y, Guo P, YuanY, Shi X, Shangguan S, Zheng H, Lai G, Huang Y, Wang Y, Song Y, Liu Y, Wu L,Wang Z, Cheng J, Wei X, Cheng M, Lai Y, Volpe G, Esteban MA, Hou Y, LiuC#, Liu L#. TranscriptomicProfile of the Mouse Postnatal Liver Development by Single-Nucleus RNASequencing.Front Cell DevBiol. 2022 Apr 6;10:833392. doi: 10.3389/fcell.2022.833392. eCollection 2022.PMID: 35465320

19. Liu C, Li R, Li Y, LinX, Zhao K, Liu Q, Wang S, Yang X, Shi X, Ma Y, Pei C, Wang H, Bao W, Hui J,Yang T, Xu Z, Lai T, Berberoglu MA, Sahu SK, Esteban MA, Ma K, Fan G, Li Y, LiuS, Chen A, Xu X#, Dong Z#, LiuL#.Spatiotemporal mapping of gene expression landscapes anddevelopmental trajectories during zebrafish embryogenesis .Dev Cell.2022Apr 22:S1534-5807(22)00249-0. doi: 10.1016/j.devcel.2022.04.009. Onlineahead of print.PMID: 35512701

20. Wang Z, Zhong Y, ZhangZ, Zhou K, Huang Z, Yu H, Liu L, Liu S, Yang H, Zhou J, Fan J, Wu L#, Sun Y#. Characteristicsand Clinical Significance of T-Cell Receptor Repertoire in Hepatocellular Carcinoma. Front Immunol. 2022 Mar 16;13:847263.doi: 10.3389/fimmu.2022.847263. eCollection 2022.PMID: 35371059

21. Wang S, Xie J, Zou X,Pan T, Yu Q, Zhuang Z, Zhong Y, Zhao X, Wang Z, Li R, Lei Y, Yin J, Yuan Y, WeiX, Liu L, Liu S, Yang H, Wu L#.Single-cell multiomics reveals heterogeneous cell stateslinked to metastatic potential in liver cancer cell lines .iScience. 2022 Feb 2;25(3):103857. doi:10.1016/j.isci.2022.103857. eCollection 2022 Mar 18.PMID: 35198910

22. Shi X, Ma W, Duan S, ShiQ, Wu S, Hao S, Dong G, Li J, Song Y, Liu C, Lin X, Yuan Y, Deng Q, Xu J, BaiS, Hou Y, Liu C#, LiuL#.Single-cell transcriptional diversity of neonatal umbilicalcord blood immune cells reveals neonatal immune tolerance .Biochem Biophys Res Commun. 2022 Jun 11;608:14-22. doi:10.1016/j.bbrc.2022.03.132. Epub 2022 Mar 28.PMID: 35381424

23. Wang S, Liu Y, Li Y, LvM, Gao K, He Y, Wei W, Zhu Y, Dong X, Xu X, Li Z#, Liu L#, Liu Y#. High-ThroughputFunctional Screening of Antigen-Specific T Cells Based on Droplet Microfluidicsat a Single-Cell Level .Anal Chem. 2022 Jan18;94(2):918-926. doi: 10.1021/acs.analchem.1c03678. Epub 2021 Dec 1.PMID: 34852202

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